NCERT · Class 12 · Biology · Biotechnology: Principles and ProcessesWhich enzyme is responsible for joining the phosphodiester bonds between adjacent DNA fragments?
Step-by-Step Solution
DNA ligase is the enzyme that acts as molecular glue, sealing nicks in the sugar-phosphate backbone by forming phosphodiester bonds between adjacent nucleotides, which is crucial in recombinant DNA technology.
Detailed Options Breakdown
Option : DNA polymerase
Incorrect choice. This distractor represents a common misunderstanding of the core principles of Biotechnology: Principles and Processes.
Option 1: Restriction endonuclease
Incorrect choice. This distractor represents a common misunderstanding of the core principles of Biotechnology: Principles and Processes.
Option 2: DNA ligase (Correct Answer)
Correct choice. Refer to the step-by-step verified solution guidelines above for details.
Option 3: Alkaline phosphatase
Incorrect choice. This distractor represents a common misunderstanding of the core principles of Biotechnology: Principles and Processes.
💡 Study Guide: This question tests core syllabus concepts from Biotechnology: Principles and Processes. For formulas, key summaries, and mock exam reference guides, read the full Biotechnology: Principles and Processes Revision Notes.