MP Board · Class 10 · Science · Heredity and EvolutionExplain Mendel's dihybrid cross with the help of a checkerboard (Punnett square). What conclusions were drawn from this cross?
Step-by-Step Solution
Introduction to Dihybrid Cross\nA dihybrid cross is a breeding experiment between two organisms that are identical hybrids for two traits. Gregor Mendel performed dihybrid crosses on pea plants by crossing plants with two contrasting characteristics, such as seed shape (round vs wrinkled) and seed color (yellow vs green).
Parental Generation and F1 Generation
- Parents: A plant with round and yellow seeds (homozygous dominant, RRYY) was crossed with a plant having wrinkled and green seeds (homozygous recessive, rryy).
- Gametes: The round-yellow plant produced gametes with the 'RY' allele combination, and the wrinkled-green plant produced gametes with the 'ry' allele combination.
- F1 Generation: All offspring in the first filial generation (F1) had the genotype RrYy and expressed the dominant phenotypes, which meant all seeds were round and yellow.
F2 Generation and Punnett Square\nWhen the F1 generation plants (RrYy) were self-pollinated, Mendel observed four different phenotypes in the F2 generation. The gametes produced by F1 plants were of four types: RY, Ry, rY, and ry, each in equal proportions (25% each).
\nWhen these gametes combined randomly in a 16-square Punnett grid:
- Round and Yellow: 9 plants
- Round and Green: 3 plants
- Wrinkled and Yellow: 3 plants
- Wrinkled and Green: 1 plant \nThus, the phenotypic ratio obtained in the F2 generation was 9 : 3 : 3 : 1.
Conclusions Drawn (Law of Independent Assortment)
- Independent Inheritance: The results proved that the factors (genes) for seed shape and seed color separate independently of one another during gamete formation.
- Law of Independent Assortment: Based on these observations, Mendel formulated the Third Law of Inheritance, which states that when two pairs of traits are combined in a hybrid, segregation of one pair of characters is independent of the other pair of characters.
💡 Study Guide: This question tests core syllabus concepts from Heredity and Evolution. For formulas, key summaries, and mock exam reference guides, read the full Heredity and Evolution Revision Notes.